Reading of the day

Better improve my cell cycle knowledge!

Beautiful DNA with bit of RNA left

Beautiful things are coming out! This one should be good for BMC Genomics I guess? Or maybe a PLOS One? Who knows... All I want is a first author. One before I hit 25 if possible =)

Frustration cycle

Just to remind myself not to fall into the "frustration N give up" trap again. Been there, done that, not again.

Primer-dimers

I keep getting shit loads of primer dimer nowadays. Not really sure what's the reason behind this but I'm troubleshooting hard enough. From the pipette to the annealing temperature all seems fine! Very likely it's the problem of my primers but according to my colleagues their amplifications are just nice! Damn. Maybe I'll try some chelex today and see how does it go. Or else i'll have to try some new flanking strategies to get this gene out.

Cracking the X-ray diffraction of DoubleHelix

Ever wondered how they deciphered the structure of the double helix based on one X-ray film? Don't you hate those lecturers for not even bothered to answer this question? Well here is the answer!

Well explained and described in plain language. Who wrote this? Watson of course =)

The daily walk back to home.

Everyday I leave the lab after 8.30pm or even more. My adrenaline rush only happens after dinner and ideas and motivations start picking up that time. If there's experiment, I'll quickly work them out. If there's none bench work I'll be discussing reviews and others with the fellows and post-docs. Thanks to these bunch (partly reaffirmed by how much they earn), I had the courage to continue this path. I'm not a risk taker. Those who knows me well, will understand that I draw my plans 15 years down the road and examined every single bad outcome. I have faith that this path is the correct path for me. One reason is I'm having an extremely happy time even with the long working hour.

Here's a photo of the path I walk every night (weekends too)