Thursday, 11 April 2013


I keep getting shit loads of primer dimer nowadays. Not really sure what's the reason behind this but I'm troubleshooting hard enough. From the pipette to the annealing temperature all seems fine! Very likely it's the problem of my primers but according to my colleagues their amplifications are just nice! Damn. Maybe I'll try some chelex today and see how does it go. Or else i'll have to try some new flanking strategies to get this gene out.

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